ZH Sem Bioinfo - Stephany Orjuela

Analysis and Method development for High-Throughput DNA Methylation and Gene Expression with Applications to Colorectal Tumors

Abstract The process by which human cells become cancerous involves genetic mutations and alterations of epigenetic processes that regulate or cause changes in gene expression. The development of high-throughput sequencing technologies that generate a molecular profile of the entire human genome has enabled a better understanding of these alterations in cancer, as well as in a myriad of other diseases. The development of corresponding methodology to extract information from these increasing amounts of high-throughput data has therefore become of high priority in the scientific community. During the last 4 years, I have analyzed high-throughput DNA methylation and gene expression sequencing data obtained from human colorectal tissues, to characterize molecular changes occurring in the step-wise process of colorectal tumorigenesis. In parallel, I developed a new method to accurately detect allelic patterns in DNA methylation, and a workflow to perform a full RNA-seq analysis. (This is a practice talk for my defense next week).

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